A validated Chinese version of Exercise Dependence Scale-Revised (EDS-R) is currently lacking. Thus, this study aimed to investigate the psychometric properties of this translated scale in a Chinese sample. Following a forward–backward translation of the EDS-R, the Chinese version (EDS-C) and validated scales assessing associated constructs were administered to a large sample of habitual exercisers (N = 1447, 54.4% male) in several universities and communities in China. Confirmatory factor analysis (CFA), gender-based invariance test, internal consistency and test–retest reliability, and construct validity were completed. EDS-C confirmed the original seven-factor structure (TLI =.94, CFI =.95, RMSEA =.068, SRMR =.032). Moreover, gender-based invariance and good internal consistency (Cronbach α [0.73 to 0.93 for sub-scales]) could be demonstrated. Beyond that a moderate test–retest reliability was observed [r: 0.45–0.82]. Finally, positive associations of the EDS-C with exercise frequency and eating disorder symptoms, body image inflexibility, and generalized anxiety symptoms appeared. Based on these results, the EDS-C appears to be a psychometrically sound screening tool for exercise dependence in Chinese sample aged between 17 to 70 years.