Applied human factors engineering technology in carriage design of mass transport system (MTS) can enhance ride comfort level and reduce disorder problems for the passenger. However, scientific and systematic measurement that applied human factors engineering methodologies for carriage design is incomplete on the contemporary studies. This paper covers the scientific and systematic measurement that applied human factors engineering methodologies for sitting and stand up posture in the simulated carriage of MTS. The main contributions of this study are to achieve the posture and force analysis by the simulation of HumanCAD and the further analysis and validation by the experiments under the simulated lateral vibration for the carriage model with anthropometry and FSA pressure mapping system. The solutions and recommendations of advanced carriage design are concluded with human factors engineering analysis.