Hyperthermia is an overheating cancer treatment. The hyperthermia studies stated that when the temperature of a cell or mass is elevated to 43 °C, destruction of the mass can be observed. Manufacturing technologies and robotics advancements enable thermal engineering researchers to conduct hyperthermia experiments. This study attempted to determine the magnitude of the heat source placed inside the vein needed to achieve hyperthermia and the section that hyperthermia covered. Non-Newtonian artificial blood was adopted as the test fluid. An artificial blood circulation system was built. A small-scale resistance heat source used to mimic the heat generated by microrobots was placed inside the imitation vein to enhance the inner wall temperature of the affected section. Experiments were conducted on a 2 mm diameter imitation vein. The experimental data was used as a base to build a numerical model for vein diameters ranging from 1.5 to 6 mm. The required power inputs to achieve hyperthermia for different vein diameters were obtained. The power ranges from 0.036 to 0.203 W for diameters from 6 mm down to 1.5 mm. The research results of this study will provide important information to researchers in robotics to achieve the required heat generation.