The production of refractory metal pre-alloy powder is difficult and the related production cost is high. In this paper, NbTaTiMox series refractory high entropy alloy was prepared by laser powder bed fusion (L-PBF) with elemental mixed powders. The effects of Mo content on the morphology, microstructure, hardness, density, and compression properties of the NbTaTiMox alloy were studied. All NbTaTiMox alloys fabricated by L-PBF present a single-phase BCC. Typical dendrites are formed within the grains and the elements are enriched in the dendrite trunk and inter-dendrite region from inside to outside depends on the melting point of the different materials. It was observed that the Mo content improves significantly the hardness and compressive strength of the alloy by solution strengthening. NbTaTiMo alloy has the highest yield strength (1609 MPa), but with reduced ductility, while NbTaTiMox (x ≤ 0.5) alloy has excellent ductility (e > 80%) at room temperature. The increase in Mo content leads to the increase of cracks, pores and non-penetration defects, resulting in the loss of sustained strength & ductility and decrease in density. The results provide a basis for the design and preparation of ductile refractory high entropy alloys by L-PBF of elemental mixed powders.