Electromechanical biomonitoring is essential in human health evaluation, diseases prevention and life quality improvement. Nanogenerators (NGs) have demonstrated exceptional performances and versatility in self-powered flexible electronics including piezoelectric and electrostatic sensors. Combined with artificial intelligent (AI), five generation (5G) and internet-of-thing (IoT) technologies, the NGs-based flexible electronics are paving a new way for creating intelligent electromechanical biomonitoring systems which are also capable of analyzing, transmitting, and deciding. In this review, we cover the recent remarkable developments in monitoring electromechanical physiological signals using NGs-based flexible electronics. We begin by covering the fundamentals of NGs from the perspective of mechanisms, materials, device structures, and manufacturing methods. We then give an overview of NGs-based flexible electronics in various wearable and implantable sensing applications. Finally, the present limitations and future developing trends of this field are discussed and prospected.