The present study examined the applicability of the Yo-Yo IE2 and a modified Yo-Yo IE2 dribbling test in non-elite young basketball players. Thirty secondary school basketball players (age, 15.1 ± 1.4 years; training history, 2.9 ± 1.3 years) were invited to perform the original (2 × 20 m) and modified (2 × 17.5 m) Yo–Yo IE2 tests, and a V̇O2max test. Test–retest performance did not significantly change for the Yo-Yo IE2 (1210  395 and 1363  442 m, p > 0.05; ICC = 0.83; CV = 15.0%; d = 0.37), whereas a small test–retest effect was observed for the modified Yo-Yo IE2 test (1175  438 and 1286  479 m, p < 0.05; ICC = 0.92; CV = 12.6%; d = 0.24). V̇O2max was correlated with the original (r = 0.55; p < 0.01) and modified (r = 0.59; p < 0.01) Yo-Yo IE2 tests. Compared to junior players, senior players had better performances on the original (p > 0.05; d = 1.19, area under the ROC curve (AUC) = 0.72) and modified (p < 0.05; d = 1.19; AUC = 0.77) Yo-Yo IE2 tests. In conclusion, these two Yo-Yo IE2 tests were reproducible and valid in basketball. The modified Yo–Yo IE2 test is a novel test to investigate endurance fitness and technical skills in young non-elite basketball players.