| Residential College | false |
| Status | 已發表Published |
| Allogenic porcine transplantation of FasL+-transfected tissue engineered chondrocytes | |
| Wang S.-J.; Zhang H.-Z.; Wang Y.; Jin S.; Ding Y.X.; Ge Y.; Shen T.W.; Ge H.-L. | |
| 2007-04-08 | |
| Source Publication | Journal of Clinical Rehabilitative Tissue Engineering Research
 |
| ISSN | 16738225 |
| Volume | 11Issue:14Pages:2605-2608 |
| Abstract | Aim: To explore the cell growth features and immune rejection responses of FasL-transfected tissue engineered chondrocytes in allogenic porcine transplantation. Methods: The experiment was done in Shanghai Immunology Institute, Medical College, Shanghai Jiao Tong University from September 2002 to March 2004. 1 The chondrocytes were isolated from porcine cartilage tissue. 2 FasL chondrocytes were isolated, and pGCEN-FasL retrovirus vectors were established. PA317 cells were transfected. After G418 screening, PA317 cell clone excreted with pGCEN-FasL retrovirus particles. High-titer virus fluid was selected to infect chondrocytes in allogenic porcine. After G418 screening, FasL chondrocyte clone was obtained, amplified and cultured. 3 FasL expression in transfected chondrocytes was analyzed by FACS. The apoptosis rate of Fas Jurkat cells and activated T cells with FasL chondrocytes; induction were detected by JAM test. At the same time, chondrocytes with pGCEN retrovirus vector was as control. 4 The FasL chondrocytes mixed with Pluronic F127 were injected subcutaneously into abdomen wall in allogenic porcine. Transplanted complexes in local tissue harvested from receipted porcine at weeks 4 or 5, the chondrocytes growth features and immune rejection reaction were detected by histopathological and immunohistochemical methods. Results: 1 The expression rate of FasL on chondrocytes was 57%. 2 FasL chondrocytes could induce apoptosis of Fas cells and activated T cell. The largest rate of apoptosis of Fas cell and activated T cell were 53.41% and 30.38% (E/ T=10:1), respectively, while those in control group were 32.27% and 13.16% (E/T=10:1). 3 The structural feature of FasL chondrocytes in reformed cartilage tissue was identical with that of normal cartilage tissue, there were obvious cartilage introcession and membrane respectively. A little unregulated align was observed in reformed cartilage tissue. 4 Immunohistochemistrical staining showed that the type II collagen in reformed cartilage tissue was even and with clear form, which was identical with that of normal cartilage tissue. 5 FasL was highly expressed in chondrocytes at the 5th week. Compared with tissue-engineered FasL chondrocytes (negative control), the presence of infiltrating lymphocytes was obviously decreased around the FasL chondrocytes. A mass of infiltrating inflammatory cells appeared around chondrocytes in the control group. Conclusion: Tissue engineered chondrocytes are successfully constructed and FasL is highly expressed, which lays the experimental foundation of immune tolerance for allogenic chondrocyte transplantation. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Shanghai Jiao Tong University |
| Recommended Citation GB/T 7714 | Wang S.-J.,Zhang H.-Z.,Wang Y.,et al. Allogenic porcine transplantation of FasL+-transfected tissue engineered chondrocytes[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2007, 11(14), 2605-2608. |
| APA | Wang S.-J.., Zhang H.-Z.., Wang Y.., Jin S.., Ding Y.X.., Ge Y.., Shen T.W.., & Ge H.-L. (2007). Allogenic porcine transplantation of FasL+-transfected tissue engineered chondrocytes. Journal of Clinical Rehabilitative Tissue Engineering Research, 11(14), 2605-2608. |
| MLA | Wang S.-J.,et al."Allogenic porcine transplantation of FasL+-transfected tissue engineered chondrocytes".Journal of Clinical Rehabilitative Tissue Engineering Research 11.14(2007):2605-2608. |
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