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Development of a solid-phase extraction-enzyme-linked immunosorbent assay method with a new sorbent of multiwall carbon nanotube for the determination of estrone in water
Wang S.; Wang Y.; Huang W.; Fang G.; Duan Z.; Qiao H.; Zhang Y.
2007-08-01
Source PublicationAnalytical Letters
ISSN00032719 1532236X
Volume40Issue:12Pages:2338-2350
Abstract

A sensitive solid-phase extraction-enzyme-linked immunosorbent assay (SPE-ELISA) method was developed to analyze the estrone in environmental water. A new SPE sorbent of the multiwall carbon nanotube was tested and proved to have similar adsorbability for estrone comparing to the commercial C18 SPE. A specific polyclonal antibody for estrone (A-E1) and a broad-spectrum antibody for estrone, estradiol and estriol (A-E2) were produced. For A-E1, the limit detection of estrone was 0.04 μg/l and for A-E2 were 0.07, 0.04 and 0.2 μg/l of estrone, estradiol and estriol, respectively. Different river water samples were analyzed by ELISA and HPLC method. Copyright © Taylor & Francis Group, LLC.

KeywordEnzyme-linked Immunosorbent Assay Estrone Multiwall Carbon Nanotube Solid-phase Extraction
DOI10.1080/00032710701577559
URLView the original
Language英語English
WOS IDWOS:000250481100007
Scopus ID2-s2.0-35649003454
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Citation statistics
Document TypeJournal article
CollectionUniversity of Macau
AffiliationTianjin University of Science & Technology
Recommended Citation
GB/T 7714
Wang S.,Wang Y.,Huang W.,et al. Development of a solid-phase extraction-enzyme-linked immunosorbent assay method with a new sorbent of multiwall carbon nanotube for the determination of estrone in water[J]. Analytical Letters, 2007, 40(12), 2338-2350.
APA Wang S.., Wang Y.., Huang W.., Fang G.., Duan Z.., Qiao H.., & Zhang Y. (2007). Development of a solid-phase extraction-enzyme-linked immunosorbent assay method with a new sorbent of multiwall carbon nanotube for the determination of estrone in water. Analytical Letters, 40(12), 2338-2350.
MLA Wang S.,et al."Development of a solid-phase extraction-enzyme-linked immunosorbent assay method with a new sorbent of multiwall carbon nanotube for the determination of estrone in water".Analytical Letters 40.12(2007):2338-2350.
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