| Residential College | false |
| Status | 已發表Published |
| Association of PD-1 expression on CD4+ CD25 nt/hi CD127 lo regulatory T cells with disease progression in HIV-1 infected patients | |
| Cao Q.H.; Xue Y.L.; Wang Y. | |
| 2009-11-01 | |
| Source Publication | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
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| ISSN | 10078738 |
| Volume | 25Issue:11Pages:1020-1022 |
| Abstract | AIM: To investigate whether Programmed death-1 (PD-1) expression on peripheral CD4(+)CD25(nt/hi)CD127(lo) regulatory T cells (Treg) was associated with disease progression in HIV-1-infected patients. METHODS: Peripheral blood from 108 HIV-1-infected patients in distinct disease progression statuses and 27 healthy individuals were collected in the present investigation. PBMCs were isolated by centrifugation on Ficoll-Hypaque, followed by staining with anti-CD4-PerCP, anti-CD25-FITC, anti-CD127-PE and anti-PD-1-APC. PD-1 expression on Treg was analyzed by four-color staining flow cytometry. CD4(+) T cell absolute counts were determined using Multitest CD3/CD4/CD8/CD45 kit and plasma viral loads were detected on NucliSens EasyQ. All data were analyzed using SPSS14.0 software. RESULTS: In peripheral blood of healthy individuals, Treg expressed PD-1 at very low levels (1.72%+/-0.65%). In contrast, Treg from HIV-1-infected patients showed a significantly increased PD-1 expression (5.33%+/-2.24%, P<0.01). Moreover, AIDS patients exhibited statistically higher PD-1 expression on Treg (7.87%+/-2.23%) than newly HIV-1 infected patients (3.22%+/-1.01%, P<0.05) and patients in progression to AIDS(5.21%+/-1.72%, P<0.05). PD-1 up-regulation on Treg was closely correlated with reduced CD4(+) T cell absolute counts but elevated plasma viral load. CONCLUSION: Overall, we found that PD-1 expression on peripheral Treg was up-regulated and correlated with disease progression in HIV-1-infected patients for the first time. These findings not only extend our understanding of how Treg functions in HIV-1-infected patients but also support the notion that blocking PD-1/PD-L1 interactions may represent a potential therapeutic strategy for HIV-1-infected patients. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Shanghai Municipal Center for Disease Control and Prevention |
| Recommended Citation GB/T 7714 | Cao Q.H.,Xue Y.L.,Wang Y.. Association of PD-1 expression on CD4+ CD25 nt/hi CD127 lo regulatory T cells with disease progression in HIV-1 infected patients[J]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2009, 25(11), 1020-1022. |
| APA | Cao Q.H.., Xue Y.L.., & Wang Y. (2009). Association of PD-1 expression on CD4+ CD25 nt/hi CD127 lo regulatory T cells with disease progression in HIV-1 infected patients. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 25(11), 1020-1022. |
| MLA | Cao Q.H.,et al."Association of PD-1 expression on CD4+ CD25 nt/hi CD127 lo regulatory T cells with disease progression in HIV-1 infected patients".Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 25.11(2009):1020-1022. |
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