A hybrid hydrogel (PP) composed of Polomaxer-407 (PO) and octapeptide with amino acid sequence of KFEFKFEF (PE) was prepared to make a scaffold material incorporating PO's high and tunable mechanical strength and integrity with PE's superior bioactivity. Human adipose-derived mesenchymal stem cells (hASCs) were encapsulated into PE, PO and PP hydrogels respectively and injected subcutaneously at the dorsal neck area of nude mice. Adipose-like tissue regeneration was only observed in the mice injected with cell-encapsulated PP hydrogel. No adipose regeneration was found in the mice injected with PO or PE. Immunohistochemistry analysis with mouse anti-human nuclei monoclonal antibody demonstrated that the cells in the regenerated adipose-like tissue was originated from the injected hASCs. The growth of blood capillaries indicated that the regenerated adipose-like tissue was living tissue. In addition, human-originated cells were also found in nude mice skin. These cells were positive with mouse anti-human cells keratin antibody, suggesting that the injected hASCs migrated to the skin and differentiated into epithelial cells in vivo. © 2011 Elsevier Ltd.