UM
Residential Collegefalse
Patent NumberCN101539550B
Status已授權 Granted
用于多糖的定性与定量分析方法
Year Issued2012
2012-05-23
Application NumberCN200910031276.5
Application Date2009-04-28
Rights Holder李绍平
李绍平; 管佳
Date Available2012-05-23
Country中国 China
Subtype发明专利 Invention
Abstract

用于多糖的定性与定量分析方法:(1)多糖的酶解及验证:多糖水溶液以比色法测定含量;(2)经HPSEC-ELSD分析,比较酶解前、后多糖图谱的变化;(3)多糖酶解液中糖类成分直接HPLC分析或柱前衍生化后HPLC分析;以标准单糖、糖醛酸、双糖等为对照和以色谱峰质荷比为参比,进行糖谱色谱峰识别;其稳定的特征片段,则用作定量分析指标;(4)通过上述各步骤的独立或联合应用,构建基于结构信息的多糖酶解响应特征和多糖酶解糖谱,实现对多糖的定性、定量分析。此法可用于人参、三七、西洋参、冬虫夏草、蛹虫草、赤芝、紫芝、黄芪和当归等中药多糖的鉴别和定量测定,为多糖及其产品的质量控制提供了有效的方法。

Language中文Chinese
Open (Notice) NumberCN101539550B
IPC Classification NumberG01n30/88 ; G01n30/89 ; G01n30/02 ; G01n30/72 ; G01n21/78 ; C12q1/34
Patent Agent栗仲平
Agency南京知识律师事务所 32207
Document TypePatent
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
李绍平,管佳. 用于多糖的定性与定量分析方法. CN101539550B[P]. 2012-05-23.
APA 李绍平., & 管佳 (2009-04-28). 用于多糖的定性与定量分析方法.
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