| Residential College | false |
| Status | 即將出版Forthcoming |
| Fractionation of biologically active poly[3-(3,4-dihydroxyphenyl)glyceric acid] preparation from symphytum asperum using hpsec-malls-rid and membrane ultrafiltration methods | |
Barbakadze, Vakhtang1; Gogilashvili, Lali1; Amiranashvili, Lela1; Merlani, Maia1; Li, Shao Ping2 ; Chankvetadze, Bezhan3
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| 2021 | |
| Source Publication | Bulletin of the Georgian National Academy of Sciences
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| ISSN | 0132-1447 |
| Volume | 15Issue:2Pages:114-119 |
| Abstract | Fractionation of biologically active poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA)-preparation from Symphytum asperum, distribution of PDPGA and polysaccharides impurity using high performance size exclusion chromatography (HPSEC) coupled with multi angle laser light scattering (MALLS), refractive index detector (RID) and membrane ultrafiltration methods were carried out. Three absorption maxima λmax at 212, 236, 286 nm in the UV spectra of PDPGA-preparation in H2O, 10 mM NaOH and 100 mM NaOH were observed. Supernatants after centrifugation as well as precipitates dissolved in 100 mM NaOH were tested on the presence of carbohydrates admixture by phenol-sulfuric acid method. Polysaccharides fraction was water soluble. The solubility of PDPGA-preparation increased with increasing alkaline concentration. Two fractions of the PDPGA-preparation obtained by HPSEC analysis were fractionated by using membrane ultrafiltration method with the cut off value of 10 kDa and got the permeate and retentive fractions. Retentive fraction has high UV absorbance and contained mainly PDPGA. Sugars mainly are in permeate fraction. |
| Keyword | 4-dihydroxyphenyl)Ethylene] 4-dihydroxyphenyl)Glyceric Acid] High Performance Size Exclusion Chromatography Multi Angle Laser Light Scattering Poly[3-(3 Poly[Oxy-1-carboxy-2-(3 Refractive Index Detector Symphytum Asperum |
| URL | View the original |
| Language | 英語English |
| Scopus ID | 2-s2.0-85117766473 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES Institute of Chinese Medical Sciences THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU) |
| Affiliation | 1.I. Kutateladze Institute of Pharmacochemistry, Tbilisi State Medical University, Tbilisi, Georgia 2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, Macao 3.Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia |
| Recommended Citation GB/T 7714 | Barbakadze, Vakhtang,Gogilashvili, Lali,Amiranashvili, Lela,et al. Fractionation of biologically active poly[3-(3,4-dihydroxyphenyl)glyceric acid] preparation from symphytum asperum using hpsec-malls-rid and membrane ultrafiltration methods[J]. Bulletin of the Georgian National Academy of Sciences, 2021, 15(2), 114-119. |
| APA | Barbakadze, Vakhtang., Gogilashvili, Lali., Amiranashvili, Lela., Merlani, Maia., Li, Shao Ping., & Chankvetadze, Bezhan (2021). Fractionation of biologically active poly[3-(3,4-dihydroxyphenyl)glyceric acid] preparation from symphytum asperum using hpsec-malls-rid and membrane ultrafiltration methods. Bulletin of the Georgian National Academy of Sciences, 15(2), 114-119. |
| MLA | Barbakadze, Vakhtang,et al."Fractionation of biologically active poly[3-(3,4-dihydroxyphenyl)glyceric acid] preparation from symphytum asperum using hpsec-malls-rid and membrane ultrafiltration methods".Bulletin of the Georgian National Academy of Sciences 15.2(2021):114-119. |
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