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Conditional depletion of nuclear proteins by the Anchor Away system
Fan X.; Geisberg J.V.; Wong K.H.; Jin Y.
2011
Source PublicationCurrent Protocols in Molecular Biology
ISSN19343639 19343647
IssueSUPPL.93
Abstract

Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus. © 2011 by John Wiley & Sons, Inc.

KeywordAnchor Away Conditional Depletion Of Nuclear Proteins
DOI10.1002/0471142727.mb1310bs93
URLView the original
Language英語English
Scopus ID2-s2.0-79953282238
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Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationHarvard Medical School
Recommended Citation
GB/T 7714
Fan X.,Geisberg J.V.,Wong K.H.,et al. Conditional depletion of nuclear proteins by the Anchor Away system[J]. Current Protocols in Molecular Biology, 2011(SUPPL.93).
APA Fan X.., Geisberg J.V.., Wong K.H.., & Jin Y. (2011). Conditional depletion of nuclear proteins by the Anchor Away system. Current Protocols in Molecular Biology(SUPPL.93).
MLA Fan X.,et al."Conditional depletion of nuclear proteins by the Anchor Away system".Current Protocols in Molecular Biology .SUPPL.93(2011).
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