Multiplex Illumina sequencing using DNA barcoding.

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Status	已發表Published
	Multiplex Illumina sequencing using DNA barcoding.
	Wong K.H.; Jin Y.; Moqtaderi Z.
	2013-05-31
Source Publication	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
ISSN	19343647
Volume	Chapter 7
Abstract	The amount of sequence obtained by modern sequencing machines greatly exceeds the sequencing depth requirements of many experiments, especially those involving organisms with small genomes. In the interest of economy and efficiency, various strategies have been developed for multiplexing, in which samples are uniquely tagged with short identifying sequences known as barcodes, pooled, and then sequenced together in a single lane. The resulting combined sequence data are subsequently sorted by barcode before bioinformatic analysis. This unit contains a barcoding protocol for the preparation of up to 96 ChIP samples for multiplex sequencing in a single flow cell lane on the Illumina platform. This strategy may be extended to even larger numbers of samples and may also be generalized to other sequencing applications or sequencing platforms. © 2013 by John Wiley & Sons, Inc.
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	Harvard Medical School
Recommended Citation GB/T 7714	Wong K.H.,Jin Y.,Moqtaderi Z.. Multiplex Illumina sequencing using DNA barcoding.[J]. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], 2013, Chapter 7.
APA	Wong K.H.., Jin Y.., & Moqtaderi Z. (2013). Multiplex Illumina sequencing using DNA barcoding.. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Chapter 7.
MLA	Wong K.H.,et al."Multiplex Illumina sequencing using DNA barcoding.".Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] Chapter 7(2013).

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