| Residential College | false |
| Status | 已發表Published |
| Negative Regulatory Effect of Pleiotrophin on Expression of Schalfen3 and Schlafen4 Genes | |
Zhou, Q.; Mi, M.; Hu, Y.; Li, G. ; Wu, D.C.; Huo, Y.
| |
| 2010-11-01 | |
| Source Publication | Chinese Journal of Biologicals
 |
| ISSN | 1004-5503 |
| Pages | 1178-1181 |
| Abstract | Objective To investigate the negative regulatory effect of pleiotrophin（PTN）on the expression of Schlafen3（Slfn3）and Schlafen4 （Slfn4）genes． Methods The expressions of Slfn3 / Slfn4 genes in control cells （Pten-/ -mouse embryo fibroblasts expressingPtn gene）and Ptn-/ -cells （Pten-/ -mouse embryo fibroblasts with Ptn gene knocked down）were determined by RT-PCR andNorthern blot． The relationship between the Ptn in human malignant glioma U87MG cells as well as human breast cancer MDA-MB-231 cells and expression of Slfn3 / Slfn4 genes were analyzed． The expression of Slfn3 / Slfn4 genes in the Ptn-/ -cells, after 50 ng / mlof PTN was added into the medium and in the control cells cultured in the medium for Ptn-/ -cells, were determined． Results Slfn3 /Slfn4 genes were highly expressed in Ptn-/ -cells． In U87MG and MDA-MB-231 cells，Ptn was negatively related to the expression ofSlfn3 / Slfn4 genes． However，after PTN was added into the medium of Ptn-/ -cells，the expression of Slfn3 / Slfn4 genes was inhibited．Ptn-/ -cell medium induced the expression of Slfn3 / Slfn4 genes． Conclusion PTN showed negative regulatory effect on theexpression of Slfn3 / Slfn4 genes |
| Keyword | Pleiotrophin (PTN) Schlafen Gene expression regulation |
| URL | View the original |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 47056 |
| Document Type | Journal article |
| Collection | Faculty of Health Sciences |
| Recommended Citation GB/T 7714 | Zhou, Q.,Mi, M.,Hu, Y.,et al. Negative Regulatory Effect of Pleiotrophin on Expression of Schalfen3 and Schlafen4 Genes[J]. Chinese Journal of Biologicals, 2010, 1178-1181. |
| APA | Zhou, Q.., Mi, M.., Hu, Y.., Li, G.., Wu, D.C.., & Huo, Y. (2010). Negative Regulatory Effect of Pleiotrophin on Expression of Schalfen3 and Schlafen4 Genes. Chinese Journal of Biologicals, 1178-1181. |
| MLA | Zhou, Q.,et al."Negative Regulatory Effect of Pleiotrophin on Expression of Schalfen3 and Schlafen4 Genes".Chinese Journal of Biologicals (2010):1178-1181. |
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