Responsible gambling (RG) is a practical approach to contain potential gambling-associated harms to an acceptable level. However, few studies have proposed a theoretical framework to account for significant individual differences in RG adherence, which hinders an effective RG promotion in public. To address this missing link, the current study aims to identify psychological need factors associated with adherence to RG practices. We applied the self-determination theory (SDT) to explore the association between the satisfaction of basic psychological needs (i.e., relatedness, competency, and autonomy) and RG adherence in a probability Chinese community adult sample (N = 1002; 55.7% women and M = 44.28 years), acquired from a telephone survey conducted in the 2018 fall with a two-stage cluster random sampling method. We found that the three types of basic needs satisfaction were significantly and positively related to RG adherence via a full mediation of flourishing. The findings provide practical insights to understanding individual differences in RG adherence and designing corresponding SDT-based interventions for gambling communities globally.