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桔梗皂苷的提取與純化
Alternative TitleExtraction and purification of polysaccharides from Platycodon grandiflorum A. DC
Jia, L; Wo, XD; Lu, J.; Wang, MM; Zhu, XS
2011-04-01
Source Publication生物學雜誌
ISSN2095-1736
Pages21-24
Abstract

中藥桔梗飲片經水浸提、乙醇分步沉澱,獲得乙醇終濃度60%和80%的兩個粗多糖組分,分別命名為PPS60和PPS80。經Molish反應、Fehling試劑反應呈陽性,碘-碘化鉀反應呈陰性,說明PPS60和PPS80中均含有多糖和還原糖,但不含有澱粉。運用苯酚-硫酸法測得PPS60和PPS80的總糖含量分別為82.25%和84.17%;3,5-二硝基水楊酸(DNS)法測得還原糖含量分別為6.21%和8.05%。PPS60和PPS80經過Sevage法除蛋白、透析、濃縮和Sephrose 6B凝膠柱層析獲得兩個單一組分。這些結果為進一步研究桔梗多糖奠定了基礎。

Keyword多糖 分步沉澱 Sephrose 6b 純化
Language其他語言Others
The Source to ArticlePB_Publication
Document TypeJournal article
CollectionDEPARTMENT OF COMMUNICATION
Corresponding AuthorLu, J.
Recommended Citation
GB/T 7714
Jia, L,Wo, XD,Lu, J.,等. 桔梗皂苷的提取與純化[J]. 生物學雜誌, 2011, 21-24.
APA Jia, L., Wo, XD., Lu, J.., Wang, MM., & Zhu, XS (2011). 桔梗皂苷的提取與純化. 生物學雜誌, 21-24.
MLA Jia, L,et al."桔梗皂苷的提取與純化".生物學雜誌 (2011):21-24.
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