Residential College | false |
Status | 已發表Published |
N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies | |
Li D.2; Liu J.4; Zhang L.4; Xu T.4; Chen J.4; Wang L.3; Zhao Q.4 | |
2015-12-01 | |
Source Publication | Virologica Sinica |
ISSN | 1995820X 16740769 |
Volume | 30Issue:6Pages:449-456 |
Abstract | The Human immunodeficiency virus type 1 (HIV-1) gp41 membrane proximal external region (MPER) is targeted by broadly neutralizing antibodies (e.g. 2F5, 4E10, Z13e and m66.6), which makes this region a promising target for vaccine design. One strategy to elicit neutralizing antibodies against the MPER epitope is to design peptide immunogens mimicking neutralization structures. To probe 2F5-like neutralizing antibodies, two yeast-displayed antibody libraries from peripheral blood mononuclear cells from a HIV-1 patient were screened against the 2F5 epitope peptide SP62. Two 2F5-like antibodies were identified that specifically recognized SP62. However, these antibodies only weakly neutralized HIV-1 primary isolates. The epitopes recognized by these two 2F5-like antibodies include not only the 2F5 epitope (amino acids (aa) 662–667 in the MPER) but also several other residues (aa 652–655) locating at the N-terminus in SP62. Experimental results suggest that residues of SP62 adjacent to the 2F5 epitope influence the response of broadly neutralizing 2F5-like antibodies in vaccination. Our findings may aid the design of vaccine immunogens and development of therapeutics against HIV-1 infection.[Figure not available: see fulltext.] |
Keyword | 2f5 Hiv-1 Membrane Proximal External Region (Mper) Neutralizing Antibody Yeast Display |
DOI | 10.1007/s12250-015-3664-6 |
URL | View the original |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Virology |
WOS Subject | Virology |
WOS ID | WOS:000367520100007 |
Scopus ID | 2-s2.0-84952024427 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Faculty of Health Sciences |
Affiliation | 1.Universidade de Macau 2.Xiamen University 3.Jilin University 4.Chinese Academy of Sciences |
Recommended Citation GB/T 7714 | Li D.,Liu J.,Zhang L.,et al. N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies[J]. Virologica Sinica, 2015, 30(6), 449-456. |
APA | Li D.., Liu J.., Zhang L.., Xu T.., Chen J.., Wang L.., & Zhao Q. (2015). N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies. Virologica Sinica, 30(6), 449-456. |
MLA | Li D.,et al."N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies".Virologica Sinica 30.6(2015):449-456. |
Files in This Item: | There are no files associated with this item. |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment