| Status | 已發表Published |
| 3D Microstructures on Digital Microfluidic Chip for Single Cell Culture | |
Zhai, J; Wong, H. H.; Li, H; Dong, C; Chuxia, D.; Jia, Y. ; Mak, P. I.; Martins, R. P.
| |
| 2018-06-24 | |
| Source Publication | 9th Asia-Pacific Conference of Transducers and Micro-Nano Technology 2018
 |
| Abstract | Precision medicine requires drug sensitivity test before therapeutic prescription to individual cancer patient. The limited cell number from biopsied human primary tumor has directed the research to microfluidic platform for drug screening [1]. However, the existence of normal cells in the population of tumor cells from the biopsy samples has become a notorious issue for data analysis. The effect on the minority cells can be obscured by the large population and the crowded cell-growth raises the difficulty in automatic cell identification. Hence, it is urged to develop an effective single-cell analysis method. Single cell culture has been investigated with channel- based droplet microfluidics for precise cell identification [2]. However, in most of these studies, the cells were exposed to a limited number of stimuli and there are possibilities that products may diffuse away. With the advantage of each droplet isolated from its surroundings, simultaneous control of many reagents, and being easily integrated with other analytical techniques, digital microfluidics (DMF) has attracted attention from scientists in recent years [3]. Cell culture and cell based analysis have been explored, but there is still no report for single cell culture on digital microfluidic (DMF) chip. In this report, a digital microfluidic system is designed for cell culture on-chip for drug screening. Fences are fabricated to form virtual channels and virtual chambers restricting the droplets in place (Fig. 1). Cells and drugs are introduced through the inlet holes and mixed on-chip with desired drug concentration before being transported to the virtual chambers for culturing. 3D microstructures are fabricated on the virtual chamber electrode to capture single cells with high efficiency and isolate the trapped cells from aggregating during the long-term culturing for single cell analysis (Fig. 2). Using three clinically established chemotherapeutic reagents, we have demonstrated that the designed structures were effective for single cell monitoring and evaluation over time. Due to the simplicity of 3D microstructure fabrication on a DMF chip, droplet controllability, and high single- cell trapping efficiency, the developed method would have great applications in precision medicine and drug response analysis at the single cell level. |
| Keyword | digital microfluidics cell culture single cell microfabrication |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 36745 |
| Document Type | Conference paper |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Jia, Y. |
| Recommended Citation GB/T 7714 | Zhai, J,Wong, H. H.,Li, H,et al. 3D Microstructures on Digital Microfluidic Chip for Single Cell Culture[C], 2018. |
| APA | Zhai, J., Wong, H. H.., Li, H., Dong, C., Chuxia, D.., Jia, Y.., Mak, P. I.., & Martins, R. P. (2018). 3D Microstructures on Digital Microfluidic Chip for Single Cell Culture. 9th Asia-Pacific Conference of Transducers and Micro-Nano Technology 2018. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment