The canonical TGF-beta signalling pathway involves Smad transcription factors through direct serine phosphorylation of the carboxy termini, nuclear translocation and regulation of transcription by receptor-regulated (R)-Smad complexes. Smads can also be phosphorylated in the linker region most prominently by the action of mitogen-activated protein (MAP) kinases, which in turn have been activated by TGF-beta or a multitude of other growth factors and hormones. Linker regionphosphorylation can prevent nuclear translocation of Smads and inhibit TGF-beta signalling, potentially leading to oncogenesis. However, some evidence has revealed that linker regionphosphorylated Smads can be translocated to the nucleus where they regulate transcription particularly of the synthesis of extracellular matrix molecules. Matrix molecules such as collagen and proteoglycans are involved in diseases such a fibrosis and atherosclerosis, respectively, and theinvolvement of linker region phosphorylation may represent a new therapeutic target.