Four novel isomers of norlignan glycoside were isolated from Cephalotaxus oliveri Mast. Their structures were elucidated as 3S-4″-O-β-D-glucopyranosylnyasol 1, 3S-4′-O-β-D-glucopyranosylnyasol 2, 3S-4″-O-β-D-glucopyranosylhinokiresinol 3, 3S-4′-O-β-D-glucopyranosylhinokiresinol 4 by extensive spectroscopic methods including 1D and 2D NMR experiments (H, C, DEPT, H–H COSY, HSQC, HMBC, ROESY) along with HR-ESIMS and comparison to literature data. Their absolute configurations were elucidated through CD spectra coupled with the quantum chemical CD calculations.