| Residential College | false |
| Status | 已發表Published |
| Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells | |
| Li J.-H.1; He C.-W.1; Liang N.-C.1; Mo L.-E.2; Zhang X.1 | |
| 1999-07-10 | |
| Source Publication | Acta Pharmacologica Sinica
 |
| ISSN | 02539756 |
| Volume | 20Issue:6Pages:541-545 |
| Abstract | AIM: To study the effect of the antitumor compounds 5F, 6F, and A from Pteris semipinnata L on the activities of DNA topoisomerases and cell cycle of HL-60 cells, and the synergism of compound 6F in combination with genistein in vitro. METHODS: DNA topoisomerases were isolated from HL-60 cell lines, and supercoiled pBR322 DNA was used as substrate to determine the activities of DNA topoisomerase I and II. Cell cycle was analyzed by flow cytometry (FCM). Cytotoxicity assay was tested by MTT method. RESULTS: Compounds 5F, 6F, and A inhibited the activities of DNA topoisomerase I and II. After exposure of the cells to compound 6F, an increase in cells in the S and G/M phases and a decrease in cells in the G/G phase of the cell cycle were observed. At low concentrations (57.8 and 115.6 nmol · L), compound 6F enhanced the cytotoxicity against HL-60 cell line in combination with genistein, q values were > 1.15. The enhancement times of 57.8 and 115.6 nmol · L of 6F by genistein were 2.60 and 4.65, respectively. CONCLUSION: Compounds 5F, 6F, and A inhibited the activities of DNA topoisomerases of HL-60 cells. Compound 6F increased the number of cells in S and G/M phases, decreased the population of G/G phase cells, and enhanced the cytotoxicity of genistein, which had synergism with 6F in antitumor action. |
| Keyword | Antineoplastic agents Cell cycle Diterpenes DNA topoisomerase Drag synergism Genistein HL-60 cells Pteris semipinnata L |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.Institute of Medical Biochemistry 2.Guangdong Medical University |
| Recommended Citation GB/T 7714 | Li J.-H.,He C.-W.,Liang N.-C.,et al. Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells[J]. Acta Pharmacologica Sinica, 1999, 20(6), 541-545. |
| APA | Li J.-H.., He C.-W.., Liang N.-C.., Mo L.-E.., & Zhang X. (1999). Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells. Acta Pharmacologica Sinica, 20(6), 541-545. |
| MLA | Li J.-H.,et al."Effects of antitumor compounds isolated from Pteris semipinnata L on DNA topoisomerases and cell cycle of HL-60 cells".Acta Pharmacologica Sinica 20.6(1999):541-545. |
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