Effects of IL-2R antisense RNA expression plasmids' transfection on the proliferation of mouse splenocytes in vitro

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	Effects of IL-2R antisense RNA expression plasmids' transfection on the proliferation of mouse splenocytes in vitro
	He C.-W.; Liang N.-C.; Zhu Z.-Y.; He X.-S.; Huang J.-F.; Ma J.-Q.
	2002
Source Publication	Chinese Pharmacological Bulletin
ISSN	10011978
Volume	18 Issue:2 Pages:139-143
Abstract	AIM: To investigate the effects of tranfection of IL-2R antisense RNA expression plasmids on mouse spleen cells' proliferation in vitro and its possible mechanism. METHODS: Spleen cells were transfected with IL-2R antisense RNA eukaryotic expression plasmids using adhesion-assisted lipofection method, and then the spleen cells were stimulated by mitogen. Cells' proliferation was tested by tetrazolium salt (MTT) method. IL-2R mRNA and protein expression level were measured by slot-blot hybridization assay and flow cytometry method respectively. RESULTS: The proliferation of spleen cells was inhibited obviously after transfecting with recombinant plasmids. The inhibitory rate of pcAnti-mlL-2Rαβ- and pciAnti-mIL-2Rαβ-transfected group was higher than that of pcAnti-mIL-2Rα-and pcAnti-mIL-2Rβ-transfected group; the inhibitory rate of pcAnti-mIL-2Rα-tranfected group was higher than that of pcAnti-mlL-2Rβ-tranfected group. No inhibitory effect on the growth of NIH3T3 cells was observed when they were transfected with recombinant plasmids. IL-2R mRNA and protein expression level were decreased in spleen cells after transfection of recombinant plasmids. CONCLUSION: IL-2R antisense RNA can efficiently inhibit the proliferation of mouse spleen cells in vitro. IL-2Rαβ chimeric antisense RNA showed higher inhibitory rate than a or antisense RNA. IL-2Rα antisense RNA was more effective than β antisense RNA. It can be concluded preliminarily that the inhibitory effect of IL-2R antisense RNA was exclusively on the growth of cells functionally expressing IL-2R. The inhibitory effect on the spleen cells' proliferation was likely due to the blocking of IL-2R expression by antisense RNA.
Keyword	Antisense Interleukin 2 Proliferation Receptor RNA Spleen cell
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	Guangdong Medical University
Recommended Citation GB/T 7714	He C.-W.,Liang N.-C.,Zhu Z.-Y.,et al. Effects of IL-2R antisense RNA expression plasmids' transfection on the proliferation of mouse splenocytes in vitro[J]. Chinese Pharmacological Bulletin, 2002, 18(2), 139-143.
APA	He C.-W.., Liang N.-C.., Zhu Z.-Y.., He X.-S.., Huang J.-F.., & Ma J.-Q. (2002). Effects of IL-2R antisense RNA expression plasmids' transfection on the proliferation of mouse splenocytes in vitro. Chinese Pharmacological Bulletin, 18(2), 139-143.
MLA	He C.-W.,et al."Effects of IL-2R antisense RNA expression plasmids' transfection on the proliferation of mouse splenocytes in vitro".Chinese Pharmacological Bulletin 18.2(2002):139-143.

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