| Residential College | false |
| Status | 已發表Published |
| Inducing apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z by bcl-xL short hairpin RNA. | |
| He C.W.; Liu F.; Zhang Y.F.; Liang T.; Zhou K.Y. | |
| 2005-06-01 | |
| Source Publication | Ai zheng = Aizheng = Chinese journal of cancer
 |
| ISSN | 1000467X |
| Volume | 24Issue:6Pages:646-652 |
| Abstract | BACKGROUND & OBJECTIVE: Recent studies showed overexpression of bcl-x(L) in human nasopharyngeal carcinoma (NPC) cell line CNE-2Z; it may play a pivotal role in tumorigenesis, metastasis, and drug resistance of NPC. This study was to explore inducing effect of bcl-x(L) short hairpin RNA (shRNA) on apoptosis of CNE-2Z cells. METHODS: After transfection of recombinant plasmid pmU6-RNAi expressing bcl-x(L) shRNA, apoptotic CNE-2Z cells were detected by fluorescent staining and flow cytometry (FCM). mRNA levels of bcl-x(L), bcl-2, survivin, and caspase-3 was detected by reverse transcription-polymerase chain reaction (RT-PCR); while protein levels of Bcl-x(L), Caspase-3, and P53 were detected by Western blot. RESULTS: When treated with pmU6-RNAi for 24 h, an obvious apoptotic peak of CNE-2Z cells appeared; cell shrinkage, chromatin condensation, and nuclear fragmentation were observed in most cells under fluorescent microscope. RT-PCR analysis showed that pmU6-RNAi down-regulated mRNA levels of bcl-x(L), bcl-2, and caspase-3, but had little or no effect on mRNA level of survivin; Western blot analysis showed an obvious reduction in protein levels of Bcl-x(L) and Caspase-3, and a great increase in protein level of P53. CONCLUSIONS: bcl-x(L) shRNA can induce apoptosis of CNE-2Z cells, which may be closely related to down-regulation of bcl-2, caspase-3 and p53. bcl-x(L) shRNA may be helpful for developing gene therapy for NPC. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Guangdong Medical University |
| Recommended Citation GB/T 7714 | He C.W.,Liu F.,Zhang Y.F.,et al. Inducing apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z by bcl-xL short hairpin RNA.[J]. Ai zheng = Aizheng = Chinese journal of cancer, 2005, 24(6), 646-652. |
| APA | He C.W.., Liu F.., Zhang Y.F.., Liang T.., & Zhou K.Y. (2005). Inducing apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z by bcl-xL short hairpin RNA.. Ai zheng = Aizheng = Chinese journal of cancer, 24(6), 646-652. |
| MLA | He C.W.,et al."Inducing apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z by bcl-xL short hairpin RNA.".Ai zheng = Aizheng = Chinese journal of cancer 24.6(2005):646-652. |
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