Role of protein kinase C α in nerve growth factor-induced arachidonic acid release from PC12 cells

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	Role of protein kinase C α in nerve growth factor-induced arachidonic acid release from PC12 cells
	Zheng W.-H.1; Fink Jr. D.W.1; Guroff G.1
	1996-05-01
Source Publication	Journal of Neurochemistry
ISSN	00223042
Volume	66 Issue:5 Pages:1868-1875
Abstract	Nerve growth factor (NGF) increases arachidonic acid (AA) release by PC12 pheochromocytoma cells. To explore the role of protein kinase C (PKC) in this action of NGF, PKC was down-regulated by long-term treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Such prolonged exposure to PMA (1 μM) resulted in the inhibition of NGF-induced AA release. Moreover, pretreatment of PC12 cells with the protein kinase inhibitor staurosporine or with calphostin C, a specific inhibitor of PKC, also blocks the increase of AA release induced by NGF. These data, as well as that PMA alone can induce AA release in PC12 cells, suggest that PKC is necessary for NGF-induced AA release. Immunoblot analysis of whole cell lysates by using antibodies against various PKC isoforms revealed that our PC12 cells contained PKCs α, β, γ, and ζ. PMA down-regulation depleted PKCs α, β, and γ, and partially depleted ζ. To see which isoform was involved in NGF-induced AA release, an isoform-specific PKC inhibitor was used. GO 6976, a compound that inhibits PKCs α and β specifically, blocked NGF-induced AA release. In addition, thymeleatoxin, a specific activator of PKCs α, β, and γ, induced AA release from PC12 cells in amounts comparable with those seen with NGF. Taken together, these data suggest that PKC α plays a role in NGF-induced AA release.
Keyword	Arachidonic acid Nerve growth factor PC12 cells Protein kinase C
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	1.National Institute of Child Health and Human Development 2.Food and Drug Administration
Recommended Citation GB/T 7714	Zheng W.-H.,Fink Jr. D.W.,Guroff G.. Role of protein kinase C α in nerve growth factor-induced arachidonic acid release from PC12 cells[J]. Journal of Neurochemistry, 1996, 66(5), 1868-1875.
APA	Zheng W.-H.., Fink Jr. D.W.., & Guroff G. (1996). Role of protein kinase C α in nerve growth factor-induced arachidonic acid release from PC12 cells. Journal of Neurochemistry, 66(5), 1868-1875.
MLA	Zheng W.-H.,et al."Role of protein kinase C α in nerve growth factor-induced arachidonic acid release from PC12 cells".Journal of Neurochemistry 66.5(1996):1868-1875.

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