| Residential College | false |
| Status | 已發表Published |
| Screening and identification of four PAHs-biodegrading strains | |
| Wang C.1; Li D.2; Liu S.1 | |
| 2007-08-25 | |
| Source Publication | Chinese Journal of Applied and Environmental Biology
 |
| ISSN | 1006687X |
| Volume | 13Issue:4Pages:546-550 |
| Abstract | Microbes play an important role in bio-remediation technologies, and it is a basic study to find out effectively functional strains for environmental bio-remediation. In this paper four PAHs-biodegrading strains were screened with a selective medium of PAHs from oily wastewater and oil-contaminated soil. The four strains were identified by their morphological, physiological and biochemical characteristics and gene analyses, and they were named as 3-28, NF, EW and EY. The 16S rDNA of the strains was amplified by PCR, indicating that they possessed 581, 582, 1209, 1 230 bp, respectively, which were compared with those available in the GenBank databases. The results showed that the 16S rDNA sequence of 3-28 strain was mostly similar to that of Microbacterium esteraromaticum, NF similar to Cellulosimicrobium cellulans, EW to Chelatococcus asacharovorans and EY to Sphingopyxis terrae, and their sequence similarities were 100%, 97.8%, 98.2% and 99.0%, respectively. The phylogenetic trees of the four strains were derived with DNAMAN and analyzed with Bootstraping. The results demonstrate that the 3-28, NF, EW and EY strains belonged to Microbacterium sp., Cellulosimicrobium sp., Chelatococcus sp. and Sphingopyxis sp., respectively. Fig 3, Tab 1, Ref 22. |
| Keyword | 16S rDNA Biodegradation Polycyclic aromatic hydrocarbons (PAHs) Sequence similarity |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.Sichuan University 2.Chengdu Institute of Biology Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang C.,Li D.,Liu S.. Screening and identification of four PAHs-biodegrading strains[J]. Chinese Journal of Applied and Environmental Biology, 2007, 13(4), 546-550. |
| APA | Wang C.., Li D.., & Liu S. (2007). Screening and identification of four PAHs-biodegrading strains. Chinese Journal of Applied and Environmental Biology, 13(4), 546-550. |
| MLA | Wang C.,et al."Screening and identification of four PAHs-biodegrading strains".Chinese Journal of Applied and Environmental Biology 13.4(2007):546-550. |
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