| Residential College | false |
| Status | 已發表Published |
| A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity | |
Leung K.-H.1; He H.-Z.1; Zhong H.-J.2; Lu L.1; Chan D.S.H.1; Ma D.-L.1 ; Leung C.-H.2
| |
| 2013-12-15 | |
| Source Publication | Methods
 |
| ISSN | 10462023 10959130 |
| Volume | 64Issue:3Pages:224-228 |
| Abstract | We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes. |
| Keyword | G-quadruplex Iridium(Iii) Complex Luminescent Polymerase Proofreading |
| DOI | 10.1016/j.ymeth.2013.05.017 |
| URL | View the original |
| Indexed By | SCIE |
| Language | 英語English |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| WOS ID | WOS:000328309600007 |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 |
| Scopus ID | 2-s2.0-84888247355 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Ma D.-L.; Leung C.-H. |
| Affiliation | 1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China 2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China |
| Corresponding Author Affilication | Institute of Chinese Medical Sciences |
| Recommended Citation GB/T 7714 | Leung K.-H.,He H.-Z.,Zhong H.-J.,et al. A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity[J]. Methods, 2013, 64(3), 224-228. |
| APA | Leung K.-H.., He H.-Z.., Zhong H.-J.., Lu L.., Chan D.S.H.., Ma D.-L.., & Leung C.-H. (2013). A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity. Methods, 64(3), 224-228. |
| MLA | Leung K.-H.,et al."A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity".Methods 64.3(2013):224-228. |
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