The roots of Stemona tuberosa have been used as an antitussive and insecticide remedy for thousands of years. On the chemical investigation of the roots of S. tuberosa, two new tuberostemonine-type alkaloids, epoxy-tuberostemonol (1), and neotuberostemoenone (2), together with a known alkaloid, neotuberostemonine, were isolated and identified. The structures of new alkaloids were established on the basis of 1D and 2D NMR and other spectroscopic analyses. © 2014 ACG Publications. All rights reserved.