Traction transformers are one of the most important equipment in the traction power supply system. Thermal aging of transformer insulation system determines its reliable life. To achieve an estimation of the insulation life of traction power transformers operated under real load condition, the effects of traction loads on the thermal aging of oil-paper insulation in traction transformers were investigated. First, the design principles for the accelerated thermal aging experiment were studied and proposed by considering magnitude (i.e., load rate) and frequency (i.e. spare time and lasting time) of the traction load. Then, an experimental system was established in the laboratory to conduct extensive accelerated thermal aging experiments, in which the traction loads were mimicked by using positive temperature coefficient (PTC) heating plates and a programmable logic controller (PLC). Finally, the experimental results were analyzed and discussed to reveal the effects of traction load on the thermal aging of oil-paper insulation in traction transformers.