An optical fiber temperature sensor based on Mach–Zehnder interferometer and thermo-optic effect of the liquid crystal (LC) in fiber ring laser (FRL) system is proposed and experimentally demonstrated. The LC is infiltrated into the core of hollow core fiber, and the resonant wavelength is more sensitive to temperature variation due to the interaction between the incident light and the cavity infiltrating liquid crystal with high thermal light coefficient. Meanwhile, the FRL system was further used to make the sensor have good performance in the case of high signal-to-noise ratio (∼35 dB), narrow half-height width (FWHM = 0.15 nm), and high sensitivity in the temperature range from 20C to 50C, with the maximum sensitivity of 1.318 nm/C. As far as we know, in the FRL system, the liquid crystal material has a better temperature sensing performance than the previous fiber. Nevertheless, the system has the advantages of good repeatability, low cost, simple production, small volume, high sensitivity. In marine microbial culture and detection, it is necessary to carry out high sensitivity measurement within a small temperature variation range. This reliable and excellent temperature performance has a potential application prospect.