This paper presents a new automatic cell bio-impedance measurement system for multiple cell condition detections. To make sure this system can work in an easy and efficient way, an effective impedance detection method based on on-board cell number-impedance theory was put forward. The method aimed to measure cell impedance by detecting the changes of interfaces between cells and electrodes. Cell impedance can be detected, recorded and processed by an embedded electronic circuit system. Some carefully designed tests proved that this new system remained high sensitivity, high efficiency and good precision in real-time cell impedance monitoring. It has the potential for further application in other cell sensing fields.