Digital microfluidics (DMF) experienced a substantial technical development in recent years, as a kind of microfluidic system that can realize automatic precise operation. This paper reports a novel method with a jetting electrode design for precise and controllable sample dispense based on EWOD force and “jetting” phenomenon. The mechanism and law of jetting were explored. We demonstrated the technique by carrying out a number of microfluidic operations including pH titration and specific DNA identification by delivering detection probe to the sample. This work provide a simple, low-cost, controllable and easy-to-use on-chip pipetting method for sample transfer.