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A dual-heater digital microfluidic system for fast polymerase chain reaction with sloppy temperature control
Wan,Liang1,2; Chen,Tianlan1; Li,Haoran1,2; Dong,Cheng1; Jia,Yanwei1; Mak,Pui In1,2; Martins,Rui P.1,2,3
2019-10
Conference NameThe 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)
Source Publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Pages807-808
Conference Date27.10.19 — 31.10.19
Conference PlaceBasel
CountrySwitzerland
Abstract

We developed a fast polymerase chain reaction (PCR) on digital microfluidic (DMF) platform which is faster than other reported PCR on DMF which took no less than 10 min. A dual-heater micro-thermal cycler using Joule heating on a sandwich-structured DMF chip was utilized for increasing the heat transfer efficiency. A sloppy temperature control scheme was adopted instead of precise temperature control to prevent the electrode breakdown and simplify the device setting.

KeywordDigital Microfluidics Fast Polymerase Chain Reaction Sloppy Temperature Control
URLView the original
Language英語English
Scopus ID2-s2.0-85094941511
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Citation statistics
Document TypeConference paper
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
Faculty of Science and Technology
THE STATE KEY LABORATORY OF ANALOG AND MIXED-SIGNAL VLSI (UNIVERSITY OF MACAU)
INSTITUTE OF MICROELECTRONICS
Corresponding AuthorJia,Yanwei
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI,Institute of Microelectronics,University of Macau,Macao
2.Department of ECE,Faculty of Science and Technology,University of Macau,Macao
3.Instituto Superior Técnico,Universidade de Lisboa,Portugal
First Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Wan,Liang,Chen,Tianlan,Li,Haoran,et al. A dual-heater digital microfluidic system for fast polymerase chain reaction with sloppy temperature control[C], 2019, 807-808.
APA Wan,Liang., Chen,Tianlan., Li,Haoran., Dong,Cheng., Jia,Yanwei., Mak,Pui In., & Martins,Rui P. (2019). A dual-heater digital microfluidic system for fast polymerase chain reaction with sloppy temperature control. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, 807-808.
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