Objective: To separate and identify chemical constituents from Coptis chinensis. Method: The compounds were separated and purified by various chromatographic techniques. Their structures were identified on the basis of their physicochemical properties using spectral techniques such as NMR and MS. Result: Thirteen compounds were separated from ethanol extracts of C. chinensis, including seven lignans, three simple phenylpropanoids, two flavones and one phenolic acid, and identified as erythro-guaiacylglycerol-8-O-4′- (coniferyl alcohol)ether(1), threo-guaiacylglycerol-8-O-4′-(coniferyl alcohol) ether(2), (+)-pinoresinol(3), (+)-medioresinol(4), (+)-lariciresinol(5) , (+)-5′-methoxylariciresinol(6), (+)-isolariciresinol(7), chlorogenic acid(8), ferulic acid (9), Z-octadecyl caffeate(10), rhamnetin(11), wogonin(12), and vanillic acid(13). Conclusion: Compounds 1,2,4,6,10-13 were separated from the genus Coptis for the first time.