Motivation is likely a pivotal factor contributing to sports participation. In India, because of the unsupportive sports/physical education environment, girls participate less in sports and other physical activities than boys. Therefore, embedding a planned healthy environment with good social support may maximize and, consequently, increase their participation in various sports and physical activities. Considering this unabated dialogue and assuming that motivation is a pivotal need for female sports participation, this study examined the differences in motivation to participate in sports among female adolescents across three age categories: early (11-14 years old), middle (15-18 years old), and late (19-21 years old) adolescence. A total of 528 female adolescents from Assam, India participated in the study. Participation in physical education courses was the criterion for the participants, and they were invited to complete the Participation Motivation Questionnaire. A factor analysis with direct oblimin rotation using the maximum likelihood extraction method yielded a 23-item, five-factor structure that exhibited moderate internal consistency. An ANOVA revealed significant differences across all three age categories regarding all the extracted factors. Moreover, t-tests of all sub-factors identified significant differences between the two achievement levels of district-level and inter-district-level athletes. This study provides sound psychometric and comparative results that are useful for education and formative reviews in applied settings and research purposes. It also serves as a measurement tool for embedding motivation to participate in physical activities and sports among female adolescents and the development of strategic ideas to assist female adolescents from less privileged areas to experience healthy growth and a healthy lifestyle.