This study investigated the Biowin-based mathematic model for anoxic/anaerobic/aerobic-MBR process in a pilot scale wastewater treatment. Calibration of the AAO-MBR model was firstly implemented, and four parameters were adjusted such as yield coefficient for heterotrophic biomass(Y_H ), maximum specific growth rate of substrate for OHO(μ_max ), decay rate constants for heterotrophic biomass(b_H ) and for AOB(b_aerob,A ) for a better fitting between the experimental data and the model outputs. The simulating concentration of COD, NH4⁺-N, TN, NO₂^- -N and NO₃^- -N were 22.2 mg·L ^-1 , 1.63 mg·L ^-1 , 27.53 mg·L ^-1 , 23.25 mg·L ^-1 and 0.05 mg·L ^-1 , respectively. The deviation of simulating and measuring concentration of COD, NH4⁺-N, TN, NO₂^- -N and NO₃^- -N were 7%, 11%, 3% , 6% and 3%, respectively. The simulation performance of AAO-MBR model was found to fit the experimental data well.