Rice grown on arsenic (As)-contaminated paddy soil can accumulatehigh levels of As in grain, posing a potential health risk. The exploration and development of potential strategies to reduceAs accumulation in rice grains are of great importance. In this study, phosphorusand siliconfertilizers were appliedon to defang rice.ExogenousAs was added to reach 50 mg/kg, and thephosphorusand silicon were applied at 0, 20,40 mg/kg and 0,50,100 mg/kg, respectively, intwo types of methods, i.e., soil application andfoliar spray. After 2-month incubation, hybrid rice cultivar of Peiza-Taifeng (Oryza sativa) was cultivated for 125 daysto evaluate As accumulation. Irrespective of the types of fertilizer application, the application of phosphorus and/or silicon reduced the As accumulation in rice grain, accounting for 9.4% to 29.9% less (soil application) and 12.8% to 41.0% less (foliar spray) respectively as compared tograin As accumulation for the treatment without fertilizer application. Significant negative correlations were observed between grain As and silicon/As, phosphorus/As mole ratio in rice straw, respectively. According to the results of full factorial experiments, the lowest As accumulation in rice grain occurred at the treatment of phosphorus 40 mg/kgand silicon 50 mg/kg. In general, the foliar spray of fertilizer performed better in terms of reducing As accumulation in rice grain, compared to soil application.