A rotating basket contactor reactor immobilized with Phanerochaete chrysosporium on polyurethane foam cubes (1.0 × 1.0× 1.0 cm³) was developed to treat a synthetic wastewater containing 1,000 μg/L of carbamazepine. The reactor was continuously operated for 160 days under non-sterile condition. The removal efficiency for carbamazepine exceeded 90% after one month of fungal adaptation through the addition of external carbon source in the influent. Nutrients and oxygen were effectively transferred to fungi, which contributed to the excellent treatment performances of the bioreactor in the long-term continuous operation. This study would provide an effective and feasible way to use the white-rot fungus bioreactor for the removal of carbamazepine under non-sterile conditions.