The Fano resonance induced by symmetry breaking could improve the sensitivity of localized surface plasmon resonance sensors. In this work, the spectra of gold nanocrescent arrays are measured and confirmed by simulation results through the finite element method (FEM). The Fano resonance presented in the spectra could be modulated by the symmetry breaking with different waist widths, which are understood through plasmonic hybridization theory with the help of surface charge distribution. Our results indicate the Fano lineshape is generated by the coherent coupling of the quadrupole plasmon mode Qof nanohole and the antibonding plasmon mode Dof nanocrescent. Finally, the high figure of merit (FoM = 1.6-3.5) of the Q mode in the visible region illustrates this nanocrescent Fano sensor is of great value in the biological and chemical scientific fields.