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Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation
Jiang, Bin; Yan, Li; Miao, Zhengqiang; Li, Enqin; Wong, Koon Ho; Xu, Ren-He
2017-07
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume133Pages:275-286
Abstract

Human stem cells are vulnerable to unfavorable conditions, and their transportation relies on costly and inconvenient cryopreservation. We report here that human mesenchymal stem cells (MSC) in spheroids survived ambient conditions (AC) many days longer than in monolayer. Under AC, the viability of MSC in spheroids remained >90% even after seven days, whereas MSC in monolayer mostly died fast. AC-exposed MSC spheroids, after recovery under normal monolayer culture conditions with controlled carbon dioxide and humidity contents, resumed typical morphology and proliferation, and retained differentiating and immunosuppressive capabilities. RNA-sequencing and other assays demonstrate that reduced cell metabolism and proliferation correlates to the enhanced survival of AC-exposed MSC in spheroids versus monolayer. Moreover, AC-exposed MSC, when injected as either single cells or spheroids, retained therapeutic effects in vivo in mouse colitis models. Spheroidal formation also prolonged survival and sustained pluripotency of human embryonic stem cells kept under AC. Therefore, this work offers an alternative and relatively simple method termed spheropreservation versus the conventional method cryopreservation. It shall remarkably simplify long-distance transportation of stem cells of these and probably also other types within temperature-mild areas, and facilitate therapeutic application of MSC as spheroids without further processing. 

KeywordStem Cells Spheroids Ambient Hypothermia Survival
DOI10.1016/j.biomaterials.2017.03.050
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, bioMaterials
WOS IDWOS:000401716700024
PublisherELSEVIER SCI LTD
The Source to ArticleWOS
Scopus ID2-s2.0-85018256759
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorXu, Ren-He
AffiliationFaculty of Health Sciences, University of Macau, Taipa, Macau
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Jiang, Bin,Yan, Li,Miao, Zhengqiang,et al. Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation[J]. BIOMATERIALS, 2017, 133, 275-286.
APA Jiang, Bin., Yan, Li., Miao, Zhengqiang., Li, Enqin., Wong, Koon Ho., & Xu, Ren-He (2017). Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation. BIOMATERIALS, 133, 275-286.
MLA Jiang, Bin,et al."Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation".BIOMATERIALS 133(2017):275-286.
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