Residential College | false |
Status | 已發表Published |
Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation | |
Jiang, Bin; Yan, Li; Miao, Zhengqiang; Li, Enqin; Wong, Koon Ho; Xu, Ren-He | |
2017-07 | |
Source Publication | BIOMATERIALS |
ISSN | 0142-9612 |
Volume | 133Pages:275-286 |
Abstract | Human stem cells are vulnerable to unfavorable conditions, and their transportation relies on costly and inconvenient cryopreservation. We report here that human mesenchymal stem cells (MSC) in spheroids survived ambient conditions (AC) many days longer than in monolayer. Under AC, the viability of MSC in spheroids remained >90% even after seven days, whereas MSC in monolayer mostly died fast. AC-exposed MSC spheroids, after recovery under normal monolayer culture conditions with controlled carbon dioxide and humidity contents, resumed typical morphology and proliferation, and retained differentiating and immunosuppressive capabilities. RNA-sequencing and other assays demonstrate that reduced cell metabolism and proliferation correlates to the enhanced survival of AC-exposed MSC in spheroids versus monolayer. Moreover, AC-exposed MSC, when injected as either single cells or spheroids, retained therapeutic effects in vivo in mouse colitis models. Spheroidal formation also prolonged survival and sustained pluripotency of human embryonic stem cells kept under AC. Therefore, this work offers an alternative and relatively simple method termed spheropreservation versus the conventional method cryopreservation. It shall remarkably simplify long-distance transportation of stem cells of these and probably also other types within temperature-mild areas, and facilitate therapeutic application of MSC as spheroids without further processing. |
Keyword | Stem Cells Spheroids Ambient Hypothermia Survival |
DOI | 10.1016/j.biomaterials.2017.03.050 |
URL | View the original |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Engineering ; Materials Science |
WOS Subject | Engineering, Biomedical ; Materials Science, bioMaterials |
WOS ID | WOS:000401716700024 |
Publisher | ELSEVIER SCI LTD |
The Source to Article | WOS |
Scopus ID | 2-s2.0-85018256759 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Faculty of Health Sciences |
Corresponding Author | Xu, Ren-He |
Affiliation | Faculty of Health Sciences, University of Macau, Taipa, Macau |
First Author Affilication | Faculty of Health Sciences |
Corresponding Author Affilication | Faculty of Health Sciences |
Recommended Citation GB/T 7714 | Jiang, Bin,Yan, Li,Miao, Zhengqiang,et al. Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation[J]. BIOMATERIALS, 2017, 133, 275-286. |
APA | Jiang, Bin., Yan, Li., Miao, Zhengqiang., Li, Enqin., Wong, Koon Ho., & Xu, Ren-He (2017). Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation. BIOMATERIALS, 133, 275-286. |
MLA | Jiang, Bin,et al."Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation".BIOMATERIALS 133(2017):275-286. |
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