Digital microfluidics (DMF) is an emerging technology in the microfluidic field to manipulate individual droplet on an electrodes array by electro-wetting forces.1 Its feature of electric driving and miniature footprint has promised it as a perfect technology for point-of-care (POC) diagnostics.2 In molecular diagnostics, Loopmediated isothermal amplification (LAMP) works at a constant temperature,3 significantly simplifies the requirement on reaction equipment. However, the widely accepted DNA detection using a double-stranded DNA dye after LAMP is less specific, given the possibility of primer-dimer formation.