Prompt point-of-care (POC) diagnostic tests based on amplification of pathogen DNAs are critical to effective treatment and control of infectious diseases. LoopMediated Isothermal Amplification (LAMP) is a potential powerful method for POC disease diagnostics as it is easy handling and cost effective with a short result turnaround time [1]. Especially, the DNA amplification can be observed with naked eyes by adding a DNA binding dye, Sybr Green I, into the final product solution. However, opening of the cap of the amplification tube may cause DNA contamination in the ambient environment inducing false-positive diagnosis later. To solve this problem, an enclosed system with internal sample manipulation is highly demanded. Digital microfluidics is a recently merged technique to manipulate (dispense, transport, mix and separate) microliter to nano-liter droplets containing biological/chemical samples on a two-dimensional electrodes array, which can reduce sample volume, provided fast heat transfer, has high reaction rate and possesses integration capacity.