This study investigates the biological removal enhancement of Di(2-ethylhexyl) phthalate (DEHP) by Acinetobacter sp., indigenous isolate, using waste tyres. The DEHP adsoption capacity was poor (2 mg/g) and the contaminant was almost completely removed after 3 days. When tyres were added with the isolate, the colony forming units (CFUs) increased with the incubation time, while the DEHP was almost completely removed within 2 days. The obtained results indicate both DEHP and Acinetobacter sp. sorbed/attached on tyre surface and the isolate utilized DEHP as carbon source to grow. Further studies are under way to better elucidate the exact DEHP removal mechanism.