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Intelligent optoelectrowetting digital microfluidic system for real-time selective parallel manipulation of biological droplet arrays
Wang, Tianyi1,2; Zhou, Shizheng3; Liu, Xuekai1; Zeng, Jianghao1; He, Xiaohan1,2; Yu, Zhihang4; Liu, Zhiyuan5; Liu, Xiaomei1; Jin, Jing4; Zhu, Yonggang4; Shi, Liuyong1; Yan, Hong6; Zhou, Teng1
2024-12
Source PublicationLab on a Chip
ISSN1473-0197
Abstract

Optoelectrowetting technology generates virtual electrodes to manipulate droplets by projecting optical patterns onto the photoconductive layer. This method avoids the complex design of the physical circuitry of dielectricwetting chips, compensating for the inability to reconstruct the electrode. However, the current technology relies on operators to manually position the droplets, draw optical patterns, and preset the droplet movement paths. It lacks real-time feedback on droplet information and the ability for independent droplet control, which can lead to droplet miscontrol and contamination. This paper presents a combination of optoelectrowetting with deep learning algorithms, integrating software and a photoelectric detection platform, and develops an optoelectrowetting intelligent control system. First, a target detection algorithm identifies droplet characteristics in real-time and automatically generate virtual electrodes to control movement. Simultaneously, a tracking algorithm outputs trajectories and ID information for efficient droplet arrays tracking. The results show that the system can automatically control the movement and fusion of multiple droplets in parallel and realize the automatic arrangement and storage of disordered droplet arrays without any additional electrodes and sensing devices. Additionally, through the automated control of the system, the cell suspension can be precisely cultured in the specified medium according to experimental requirements, and the growth trend is consistent with that observed in the well plate, significantly enhancing the experiment's flexibility and accuracy. In this paper, we propose an intelligent method applicable to the automated manipulation of discrete droplets. This method would play a crucial role in advancing the applications of digital microfluidic technology in biomedicine and other fields.

DOI10.1039/d4lc00804a
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
WOS IDWOS:001373899500001
PublisherROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Scopus ID2-s2.0-85211975889
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Citation statistics
Document TypeJournal article
CollectionINSTITUTE OF APPLIED PHYSICS AND MATERIALS ENGINEERING
Corresponding AuthorShi, Liuyong; Zhou, Teng
Affiliation1.School of Mechanical and Electrical Engineering, Hainan University, Haikou, Hainan, 570228, China
2.School of Information and Communication Engineering, Hainan University, Haikou, Hainan, 570228, China
3.Institute of Applied Physics and Materials Engineering, University of Macau, 999078, Macao
4.Center for Microflows and Nanoflows, School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, China
5.School of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China
6.School of Computer Science and Technology, Hainan University, Haikou, Hainan, 570228, China
Recommended Citation
GB/T 7714
Wang, Tianyi,Zhou, Shizheng,Liu, Xuekai,et al. Intelligent optoelectrowetting digital microfluidic system for real-time selective parallel manipulation of biological droplet arrays[J]. Lab on a Chip, 2024.
APA Wang, Tianyi., Zhou, Shizheng., Liu, Xuekai., Zeng, Jianghao., He, Xiaohan., Yu, Zhihang., Liu, Zhiyuan., Liu, Xiaomei., Jin, Jing., Zhu, Yonggang., Shi, Liuyong., Yan, Hong., & Zhou, Teng (2024). Intelligent optoelectrowetting digital microfluidic system for real-time selective parallel manipulation of biological droplet arrays. Lab on a Chip.
MLA Wang, Tianyi,et al."Intelligent optoelectrowetting digital microfluidic system for real-time selective parallel manipulation of biological droplet arrays".Lab on a Chip (2024).
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