Bioactivity-based molecular networking guided identification of guttiferone J from Garcinia cambogia as an anti-obesity candidate

UM > Institute of Chinese Medical Sciences

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Status	已發表Published
	Bioactivity-based molecular networking guided identification of guttiferone J from Garcinia cambogia as an anti-obesity candidate
	LIN LIGEN
	2024-10
Size of Audience	80
Type of Speaker	invited talk
Abstract	Pharmacological intervention to induce browning of white adipose tissue provides a promising anti-obesity therapy. The fruits of Garcinia cambogia (Clusiaceae) have been widely applied to manage body weight, however the chemical principles remain unclear. The current study aims to discover browning inducers from the fruits of G. cambogia and investigate the underlying mechanisms. The bioactivity-based molecular networking-guided isolation yielded several polycyclic polyprenylated acylphloroglucinols from the fruits of G. cambogia with lipid lowering effect on adipocytes, including guttiferone J (GOJ), garcinol and 14-deoxygarcinol. As the most potent one, GOJ (10 µM) reduced lipid accumulation by 70% and 76% in 3T3-L1 and C3H10T1/2 adipocytes, respectively. Furthermore, GOJ (2.5‒10 µM) increased the expression of the deacetylase Sirtuin 3 (SIRT3) and activated it, which, in turn, reduced the acetylation level of PPARγ coactivator-1α to boost mitochondrial biogenesis, and promoted uncoupling protein 1 expression to enhance thermogenesis, resulting in browning of adipocytes. In high-fat diet-induced-obese mice, GOJ (10 and 20 mg/kg/day for 12 weeks) protected against adiposity, hyperlipidemia, insulin resistance and liver lipotoxicity, through boosting SIRT3-mediated browning of inguinal adipose tissue. GOJ represents a new scaffold of thermogenic inducer, which is responsible for the anti-obesity property of G. cambogia and can be further developed as a candidate for treating obesity and its related disorders.
Source Publication	THE FIFTH ANNUAL CONFERENCE OF THE PAN-BALKAN ALLIANCE OF NATURAL PRODUCTS AND DRUG DISCOVERY ASSOCIATIONS
Conference Date	22/10/2024-24/10/2024
Conference Place	Belgrade, Serbia
Document Type	Presentation
Collection	Institute of Chinese Medical Sciences
Affiliation	University of Macau
Recommended Citation GB/T 7714	LIN LIGEN. Bioactivity-based molecular networking guided identification of guttiferone J from Garcinia cambogia as an anti-obesity candidate[Z]. THE FIFTH ANNUAL CONFERENCE OF THE PAN-BALKAN ALLIANCE OF NATURAL PRODUCTS AND DRUG DISCOVERY ASSOCIATIONS, 22/10/2024-24/10/2024.

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