| Residential College | false |
| Status | 即將出版Forthcoming |
| Intercellular interactions mediated by hgf and tgf-Β promote the 3d spherical and xenograft growth of liver cancer cells | |
| Peng, Zheng1; Lv, Xiaolan2; Zhang, Pengfei3; Chen, Qiao4; Zhang, Hongyu1; Chen, Jianlin5; Ma, Xingxuan1; Ouyang, Bohui1; Hao, Meng6; Tong, Haibo6; Guo, Dongwei3; Luo, Yi7; Huang, Shigao8 | |
| 2024 | |
| Source Publication | Current Protein and Peptide Science
 |
| ISSN | 1389-2037 |
| Volume | 25Issue:1Pages:71-82 |
| Abstract | Background: Recently, the importance of the interactions between liver cancer cells and fibroblasts has been increasingly recognized; however, many details remain to be explored. Methods: In this work, we first studied their intercellular interactions using conditioned medium from mouse embryonic fibroblasts (MEFs), then through a previously established coculture model. Results: Culturing in a conditioned medium from MEFs could significantly increase the growth, migration, and invasion of liver cancer cells. The coculture model further demonstrated that a positive feedback loop was formed between transforming growth factor-β (TGF-β) from HepG2 cells and mHGF (mouse hepatocyte growth factor) from MEFs during coculture. In this feedback loop, c-Met expression in HepG2 cells was significantly increased, and its downstream signaling pathways, such as Src/FAK, PI3K/AKT, and RAF/MEK/ERK, were activated. Moreover, the proportion of activated MEFs was also increased. More importantly, the growth-promoting effects caused by the interaction of these two cell types were validated in vitro by a 3D spheroid growth assay and in vivo by a xenograft mouse model. Conclusion: Collectively, these findings provide valuable insights into the interactions between fibroblasts and liver cancer cells, which may have therapeutic implications for the treatment of liver cancer. |
| Keyword | 3D spheroid Coculture Fibroblasts Hepatocyte growth factor Liver cancer cells Transforming growth factor-β |
| DOI | 10.2174/1389203724666230825100318 |
| URL | View the original |
| Language | 英語English |
| Scopus ID | 2-s2.0-85184144618 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.Department of Clinical Laboratory, Liuzhou Traditional Chinese Medical Hospital, Liuzhou, Guangxi, China 2.Department of Clinical Laboratory, Liuzhou Maternity and Child Healthcare Hospital, Liuzhou, Guangxi, China 3.Department of Pulmonary and Critical Care Medicine, Liuzhou Traditional Chinese Medical Hospital, Liuzhou, Guangxi, China 4.Department of gastroenterology, Liuzhou Traditional Chinese Medical Hospital, Liuzhou, Guangxi, China 5.Shengli Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, 350001, China 6.Faculty of Health Sciences, University of Macau, Taipa, Macao 7.Department of Neurosurgery, Liuzhou Traditional Chinese Medical Hospital, Liuzhou, Guangxi, China 8.Department of Radiation Oncology, The First Affiliated Hospital, Air Force Medical University, Xi'an, China |
| Recommended Citation GB/T 7714 | Peng, Zheng,Lv, Xiaolan,Zhang, Pengfei,et al. Intercellular interactions mediated by hgf and tgf-Β promote the 3d spherical and xenograft growth of liver cancer cells[J]. Current Protein and Peptide Science, 2024, 25(1), 71-82. |
| APA | Peng, Zheng., Lv, Xiaolan., Zhang, Pengfei., Chen, Qiao., Zhang, Hongyu., Chen, Jianlin., Ma, Xingxuan., Ouyang, Bohui., Hao, Meng., Tong, Haibo., Guo, Dongwei., Luo, Yi., & Huang, Shigao (2024). Intercellular interactions mediated by hgf and tgf-Β promote the 3d spherical and xenograft growth of liver cancer cells. Current Protein and Peptide Science, 25(1), 71-82. |
| MLA | Peng, Zheng,et al."Intercellular interactions mediated by hgf and tgf-Β promote the 3d spherical and xenograft growth of liver cancer cells".Current Protein and Peptide Science 25.1(2024):71-82. |
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