In this communication, a novel highly integrated antenna-triplexer on a two-layer printed circuit board is proposed for multiband communication systems. This integrated three-port component operated at triple frequency bands (0.85, 1.65 and 2.45 GHz) simultaneously utilizes a single slot antenna and its rectangular loop as the radiating elements. Good three-port isolations are achieved by the multi-mode excitations including even, odd and bi-directional modes. These modes are intrinsically orthogonal and therefore the isolations are guaranteed. Compared with the conventional counterparts that use additional bandpass filters to assure the port isolations, the proposed antenna-triplexer preserves the simplicity and compactness of the antenna itself, and it is suitable for the practical portable applications. To demonstrate the significance of this new antenna-triplexer, a prototype measured with compact size is implemented and the experimental results show the good agreements with the simulations. The antenna gains of 0.85, 4.00 and 4.23 dBi are obtained and an average isolation in three ports is as good as 22.7 dB at operating frequencies.