| Residential College | false |
| Status | 已發表Published |
| Navigating chimeric antigen receptor-engineered natural killer cells as drug carriers via three-dimensional mapping of the tumor microenvironment | |
Huang Shigaao ; YUAN ZHEN ; Zhao Qi
| |
| 2024-07 | |
| Source Publication | Journal of Controlled Release
 |
| ISSN | 1873-4995 |
| Volume | 362Pages:524-535 |
| Abstract | Chimeric antigen receptor (CAR)-modified natural killer (NK) cells are recognized as promising immunotherapeutic agents for cancer treatment. However, the efficacy and trafficking of CAR-NK cells in solid tumors are hindered by the complex barriers present in the tumor microenvironment (TME). We have developed a novel strategy that utilizes living CAR-NK cells as carriers to deliver anticancer drugs specifically to the tumor site. We also introduce a time-lapse method for evaluating the efficacy and tumor specificity of CAR-NK cells using a twophoton microscope in live mouse models and three-dimensional (3D) tissue slide cultures. Our results demonstrate that CAR-NK cells exhibit enhanced antitumor immunity when combined with photosensitive chemicals in both in vitro and in vivo tumor models. Additionally, we have successfully visualized the trafficking, infiltration, and accumulation of drug-loaded CAR-NK cells in deeply situated TME using non-invasive intravital two-photon microscopy. Our findings highlight that tumor infiltration of CAR-NK cells can be intravitally monitored through the two-photon microscope approach. In conclusion, our study demonstrates the successful integration of CARNK cells as drug carriers and paves the way for combined cellular and small-molecule therapies in cancer treatment. Furthermore, our 3D platform offers a valuable tool for assessing the behavior of CAR cells within solid tumors, facilitating the development and optimization of immunotherapeutic strategies with clinical imaging approaches. |
| Indexed By | SCIE |
| Document Type | Journal article |
| Collection | DEPARTMENT OF PUBLIC HEALTH AND MEDICINAL ADMINISTRATION |
| Corresponding Author | Zhao Qi |
| Affiliation | Faculty of Health Sciences, Univ Macau |
| First Author Affilication | Faculty of Health Sciences |
| Corresponding Author Affilication | Faculty of Health Sciences |
| Recommended Citation GB/T 7714 | Huang Shigaao,YUAN ZHEN,Zhao Qi. Navigating chimeric antigen receptor-engineered natural killer cells as drug carriers via three-dimensional mapping of the tumor microenvironment[J]. Journal of Controlled Release, 2024, 362, 524-535. |
| APA | Huang Shigaao., YUAN ZHEN., & Zhao Qi (2024). Navigating chimeric antigen receptor-engineered natural killer cells as drug carriers via three-dimensional mapping of the tumor microenvironment. Journal of Controlled Release, 362, 524-535. |
| MLA | Huang Shigaao,et al."Navigating chimeric antigen receptor-engineered natural killer cells as drug carriers via three-dimensional mapping of the tumor microenvironment".Journal of Controlled Release 362(2024):524-535. |
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| 1.pdf（9616KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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