| Residential College | false |
| Status | 已發表Published |
| Natural compound RGGC ameliorates osteoporosis by modulating osteoblast differentiation and osteoclast formation | |
TAN LIHUA ; HE CHENGWEI 
| |
| 2022-12 | |
| Publisher | Internal communicationa |
| Conference Name | The 4th Sino-CPLP Symposium on Natural Medicine and Biodiversity Resources (SNMBR) & the International Forum on Research and Development of Traditional Chinese Medicine Industry (Macao) |
| Conference Place | Macau |
| Conference Date | 2022-12-02--2022-12-05 |
| Country | China |
| Abstract | Osteoporosis has become a serious public health issue influencing more than 200 million people worldwide and a huge social burden with the aging of population. Current therapeutic approaches for osteoporosis exhibit limited outcomes but many side effects. Novel effective and safe drugs for the prevention and treatment of osteoporosis are highly demanded. RGGC, a natural compound isolated from licorice, has been found to display multiple bioactivities. However, the therapeutic potential of RGGC on osteoporosis is still unclear. Hence, the aim of this study is to examine the osteoprotective effect and underlying mechanisms of RGGC in vitro and in vivo. The results of TRAP staining assay, pit-formation assay and F-actin ring formation assay revealed that RGGC strongly suppressed in vitro osteoclast formation and osteoclast function in RANKL-induced RAW264.7 cells and bone marrow macrophages (BMMs). Additionally, RGGC exhibited remarkable inhibitory action on the expression of osteoclast-specific proteins and genes (TRAP, CTSK, MMP9, c-fos, NFATc1 and OC-STAMP) via modulation of RANKL-induced Ca2+ oscillation, MAPK and RANK/RANKL signaling activation. Moreover, RGGC was also observed to stimulate osteoblast differentiation and bone mineralization, as indicated by increased activities of ALP and alizarin red‐mineralization in bone marrow stromal cells (BMSC) and mouse calvarial osteoblasts (MOBs). Consistent with the in vitro results, RGGC strongly suppressed the reduction in the alizarin red staining area and cumulative optical density of zebrafish skulls in dexamethasone-induced zebrafish osteoporosis. Taken together, RGGC exhibits osteoprotective effect by inhibiting osteoclast formation and promoting osteoblast differentiation, suggesting its potential application in the development of effective and safe medicine or dietary supplement for the prevention and treatment of osteoporosis. |
| Keyword | Natural Compound Osteoporosis Osteoclast Osteoblast Ca2++ Oscillation |
| Document Type | Conference proceedings |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | HE CHENGWEI |
| Affiliation | University of Macau |
| First Author Affilication | University of Macau |
| Recommended Citation GB/T 7714 | TAN LIHUA,HE CHENGWEI. Natural compound RGGC ameliorates osteoporosis by modulating osteoblast differentiation and osteoclast formation[C]:Internal communicationa, 2022. |
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| 2022 4th Sino-CPLP S（11124KB） | 会议录 | 开放获取 | CC BY-NC-SA | View Download | ||
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