Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

doi:10.1002/jev2.12404

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	Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
	Welsh, JA (Welsh, Joshua A.)1; Deborah C. I. Goberdhan 2; Lorraine O’Driscoll 3,4,5; Edit I. Buzas 6,7,8; Cherie Blenkiron 9; Benedetta Bussolati 10; Houjian Cai 11; Dolores Di Vizio 12; Tom A. P. Driedonks 13; Uta Erdbrügger 14; Juan M. Falcon-Perez 15,16,17; Qing-Ling Fu 18,19; Andrew F. Hill 20; Metka Lenassi 21; Sai Kiang Lim 22,23,24; M˜y G. Mahoney 25; Sujata Mohanty 26; Andreas Möller 27,28; Rienk Nieuwland 29,30; Takahiro Ochiya 31; Susmita Sahoo 32; Ana C. Torrecilhas 33; Lei Zheng 34; Andries Zijlstra 35,36; Sarah Abuelreich 37; Reem Bagabas 37; Paolo Bergese 38,39,40; Esther M. Bridges 41; Marco Brucale 42,43; Dylan Burger 44,45,46; Randy P. Carney 47; Emanuele Cocucci 48,49; Rossella Crescitelli 50,51; Edveena Hanser 52,53; Adrian L. Harris 54; Norman J. Haughey 55; An Hendrix 56,57; Alexander R. Ivanov 58; Tijana Jovanovic-Talisman 59; Nicole A. Kruh-Garcia 60; Vroniqa Ku’ulei-Lyn Faustino 37; Diego Kyburz 53,61; Cecilia Lässer 62; Kathleen M. Lennon 37; Jan Lötvall 63; Adam L. Maddox 37; Elena S. Martens-Uzunova 64; Rachel R. Mizenko 47; Lauren A. Newman 65; Andrea Ridolfi 66; Eva Rohde 67,68,69; Tatu Rojalin 47,70; Andrew Rowland 65; Andras Saftics 37; Ursula S. Sandau 71; Julie A. Saugstad 71; Faezeh Shekari 72,73; Simon Swift 74; Dmitry Ter-Ovanesyan 75; Juan P. Tosar 76,77; Zivile Useckaite 65; Francesco Valle 42,43; Zoltan Varga 78,79; Edwin van der Pol 30,80,81; Martijn J. C. vanHerwijnen 82; Marca H. M. Wauben 82; Ann M. Wehman 83; Sarah Williams 84; Andrea Zendrini 38,39; Alan J. Zimmerman 58; MISEV ConsortiumClotilde Théry 85,86; Kenneth W. Witwer 87,88,89
	2024-02
Source Publication	Journal of Extracellular Vesicles
ISSN	2001-3078
Volume	13 Issue:2 Pages:e12404
Abstract	Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. © 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
Keyword	Ectosomes Exosomes Extracellular Particles Extracellular Vesicles Guidelines Microparticles Microvesicles Minimal Information Requirements Misev Reproducibility Rigor Standardisation
DOI	10.1002/jev2.12404
URL	View the original
Indexed By	SCIE
Language	英語English
WOS Research Area	Cell Biology
WOS Subject	Cell Biology
WOS ID	WOS:001157744000001
Publisher	WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ
Scopus ID	2-s2.0-85183359756
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science View Full-Text via Scopus
Citation statistics	Cited Times [WOS]:793 [WOS Record] [Related Records in WOS]
Document Type	Journal article
Collection	Faculty of Health Sciences
Corresponding Author	Welsh, JA (Welsh, Joshua A.); Deborah C. I. Goberdhan; Lorraine O’Driscoll; MISEV ConsortiumClotilde Théry; Kenneth W. Witwer
Affiliation	1.Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA 2.Nuffield Department of Women’s and Reproductive Health, University of Oxford, Women’s Centre, John Radcliffe Hospital, Oxford, UK 3.School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland 4.Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland 5.Trinity St. James’s Cancer Institute, Trinity College Dublin, Dublin, Ireland 6.Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary 7.HCEMM-SU Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary 8.HUN-REN-SU Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary 9.Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand 10.Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy 11.University of Georgia, Athens, Georgia, USA 12.Department of Surgery, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, California, USA 13.Department CDL Research, University Medical Center Utrecht, Utrecht, The Netherlands 14.University of Virginia Health System, Charlottesville, Virginia, USA 15.Exosomes Laboratory, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain 16.Metabolomics Platform, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain 17.IKERBASQUE, Basque Foundation for Science, Bilbao, Spain 18.Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China 19.Extracellular Vesicle Research and Clinical Translational Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China 20.Institute for Health and Sport, Victoria University, Melbourne, Australia 21.Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia 22.Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore 23.Paracrine Therapeutics Pte. Ltd., Singapore, Singapore 24.Department of Surgery, YLL School of Medicine, National University Singapore, Singapore, Singapore 25.Thomas Jefferson University, Philadelphia, Pennsylvania, USA 26.Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India 27.Chinese University of Hong Kong, Hong Kong, Hong Kong S.A.R 28.QIMR Berghofer Medical Research Institute, Brisbane, Australia 29.Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands 30.Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands 31.Tokyo Medical University, Tokyo, Japan 32.Icahn School of Medicine at Mount Sinai, New York, New York, USA 33.Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas,Universidade Federal de São Paulo (UNIFESP) Campus Diadema, Diadema, Brazil 34.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China 35.Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA 36.Genentech, South San Francisco, California, USA 37.Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA 38.Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy 39.Center for Colloid and Surface Science (CSGI), Florence, Italy 40.National Center for Gene Therapy and Drugs based on RNA Technology, Padua, Italy 41.Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK 42.Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy 43.Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy 44.Kidney Research Centre, Ottawa Hopsital Research Institute, Ottawa, Canada 45.Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada 46.School of Pharmaceutical Sciences, University of Ottawa, Ottawa, Canada 47.Department of Biomedical Engineering, University of California, Davis, California, USA 48.Division of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio, USA 49.Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA 50.Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden 51.Wallenberg Centre for Molecular and Translational Medicine, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden 52.Department of Biomedicine, University Hospital Basel, Basel, Switzerland 53.Department of Biomedicine, University of Basel, Basel, Switzerland 54.Department of Oncology, University of Oxford, Oxford, UK 55.Departments of Neurology and Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 56.Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium 57.Cancer Research Institute Ghent, Ghent, Belgium 58.Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA 59.Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA 60.Bio-pharmaceutical Manufacturing and Academic Resource Center (BioMARC), Infectious Disease Research Center, Colorado State University, Fort Collins, Colorado, USA 61.Department of Rheumatology, University Hospital Basel, Basel, Switzerland 62.Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden 63.Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden 64.Erasmus MC Cancer Institute, University Medical Center Rotterdam, Department of Urology, Rotterdam, The Netherlands 65.College of Medicine and Public Health, Flinders University, Adelaide, Australia 66.Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands 67.Department of Transfusion Medicine, University Hospital, Salzburger Landeskliniken GmbH of Paracelsus Medical University, Salzburg, Austria 68.GMP Unit, Paracelsus Medical University, Salzburg, Austria 69.Transfer Centre for Extracellular Vesicle Theralytic Technologies, EV-TT, Salzburg, Austria 70.Expansion Therapeutics, Structural Biology and Biophysics, Jupiter, Florida, USA 71.Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA 72.Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran 73.Celer Diagnostics, Toronto, Canada 74.Waipapa Taumata Rau University of Auckland, Auckland, New Zealand 75.Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA 76.Universidad de la República, Montevideo, Uruguay 77.Institut Pasteur de Montevideo, Montevideo, Uruguay 78.Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary 79.Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary 80.Biomedical Engineering and Physics, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands 81.Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands 82.Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands 83.University of Denver, Denver, USA 84.International Society for Extracellular Vesicles 85.Institut Curie, INSERM U932, PSL University, Paris, France 86.CurieCoreTech Extracellular Vesicles, Institut Curie, Paris, France 87.Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 88.EV Core Facility “EXCEL”, Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 89.The Richman Family Precision Medicine Center of Excellence in Alzheimer’s Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Recommended Citation GB/T 7714	Welsh, JA ,Deborah C. I. Goberdhan,Lorraine O’Driscoll,et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches[J]. Journal of Extracellular Vesicles, 2024, 13(2), e12404.
APA	Welsh, JA ., Deborah C. I. Goberdhan., Lorraine O’Driscoll., Edit I. Buzas., Cherie Blenkiron., Benedetta Bussolati., Houjian Cai., Dolores Di Vizio., Tom A. P. Driedonks., Uta Erdbrügger., Juan M. Falcon-Perez., Qing-Ling Fu., Andrew F. Hill., Metka Lenassi., Sai Kiang Lim., M˜y G. Mahoney., Sujata Mohanty., Andreas Möller., Rienk Nieuwland., ...& Kenneth W. Witwer (2024). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles, 13(2), e12404.
MLA	Welsh, JA ,et al."Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches".Journal of Extracellular Vesicles 13.2(2024):e12404.

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